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KMID : 0985420070290020211
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Laboratory Medicine and Quality Assurance
2007 Volume.29 No. 2 p.211 ~ p.216
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Evaluation of Automated Flow Cytometric Analyzer Sysmex UF-100 for Cell Counting of Cerebrospinal Fluid
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Lee Ja-Young
Lee Jeong-Nyeo
Kim Hye-Ran
Shin Jeong-Hwan
Kim Jae-Hyun
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Abstract
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Background: The counting of erythrocyte and leukocyte in cerebrospinal fluid (CSF) is still performed microscopically, using a chamber in most laboratories. However, it is imprecise, has wide inter-observer variability, and is labor-intensive and time-consuming. This study was aimed to examine the possibility of using Sysmex UF-100 flow cytometry (Toa Medical Electronics, Japan) as a means of counting cells in CSF samples.
Methods: From May to September 2006, we obtained 115 routinely collected CSF samples from 90 patients. We compared the performance of the automated method of the UF-100 with the manual counting method using Neubauer chamber.
Results: Accuracy statistics for erythrocyte and leukocyte showed a high correlation between the UF-100 and the manual counting method, with correlation coefficients of r2=0.95 and 0.89, respectively. Linearity results demonstrated that the UF-100 method provides accurate results throughout the reportable ranges of erythrocyte and leukocytes. A high degree of inter-assay precision for the UF-100 method was seen. Five cases with high lymphocytes percentage showed falsely low value of leukocyte counts.
Conclusions: The flow cytometric analysis of CSF with the UF-100 offered a rapid and reliable erythrocyte and leukocyte count. UF-100 is expected to be useful for screening method in CSF cell counting. But the manual counting method is still needed for the samples with high leukocyte count or contaminated with peripheral bloods.
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KEYWORD
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Cerebrospinal fluid, UF-100, Urine flow cytometer, Chamber counting
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